---
title: "Layout Plot"
output: rmarkdown::html_vignette
vignette: >
%\VignetteIndexEntry{Plot Integration}
%\VignetteEngine{knitr::rmarkdown}
%\VignetteEncoding{UTF-8}
---
```{r, include = FALSE}
knitr::opts_chunk$set(
collapse = TRUE,
comment = "#>"
)
```
The `ggalign` package offers several `align_*` functions that allow you to
precisely control plot layout and integrate additional plots. Currently, there
are two primary `align_*` functions for adding plots:
- `align_gg()`/`ggalign()`: Create ggplot object with a customized data.
- `align_panel()`/`ggpanel()`: Create ggplot object with the layout panel
data.
```{r setup}
library(ggalign)
```
```{r setup_data}
set.seed(123)
small_mat <- matrix(rnorm(81), nrow = 9)
rownames(small_mat) <- paste0("row", seq_len(nrow(small_mat)))
colnames(small_mat) <- paste0("column", seq_len(ncol(small_mat)))
```
## `align_gg`
`align_gg()` is similar to `ggplot` in that it initializes a `ggplot` data and
`mapping`. Same with other `align_*` functions. `align_gg()` allowing you to
provide data in various formats, including matrices, data frames, or simple
vectors. This data can be also inherited from the layout.
`align_gg()` always applies a default mapping for the parallel axes of the data
index within the layout. This mapping is `aes(y = .data$.y)` for horizontal
stacking (including left and right heatmap annotation) and `aes(x = .data$.x)`
for vertical stacking (including top and bottom heatmap annotation). For more
information, refer to the "ggplot2 Specification" section in the `align_gg()`
documentation.
> Note: All `align_*` functions treat rows as observations, meaning the `NROW()`
function must return a number equal to the parallel layout axis. For heatmap
column annotations, the heatmap matrix is transposed before being used. If data
is a function, it will be applied to the transposed matrix. In this example, we
use `rowSums()` to calculate the sum value across all columns.
You can also use the `ggalign()` function, which is an alias for `align_gg()`.
```{r}
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t") +
ggalign(data = rowSums) +
geom_point(aes(y = value))
```
See `ggplot2 specification` in `align_gg()`/`ggalign()` for the data details.
### Plot data
`align_gg()`/`ggalign()` requires the specific data format for its operations.
If you need to transform or filter data for individual `geoms`, you can use the
`data` argument within each `geom`. However, if you have multiple `geoms` and
want a consistent transformation applied across all, you can utilize the
`plot_data` argument in the `align_gg()`/`ggalign()` function. This allows you
to transform the default data for all subsequent geoms.
```{r}
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t") +
align_kmeans(3L) +
ggalign(plot_data = function(data) subset(data, .panel == 1L)) +
geom_bar(aes(y = value, fill = .row_names), stat = "identity")
```
### Cross panel sumamry
When used in a heatmap layout, and the data is inherited from the heatmap data,
a special column `.extra_panel` will be added, which is the panel information
for column (left or right annotation) or row (top or bottom annotation). This is
useful if you want to create summary plot using another axis panel. In such
cases, it's often necessary to disable the automatic setting of limits (`limits
= FALSE` in `align_gg()`).
```{r fig.dim = c(5, 10)}
set.seed(1L)
v <- stats::rnorm(50L)
split <- sample(letters[1:2], 50L, replace = TRUE)
ggheatmap(v) +
scale_fill_viridis_c() +
theme(strip.text = element_text(), strip.background = element_rect()) +
hmanno("r") +
align_group(split) +
hmanno("t", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(.extra_panel, value, fill = .extra_panel),
# here, we use `print()` to show the underlying data
data = function(data) {
print(head(data))
data
}
) +
# the default will always use continuous scale, here, we override it, since
# the panel is a factor variable, we should use discrete scale.
scale_x_discrete() +
scale_fill_brewer(palette = "Dark2", name = "branch")
```
This approach replicates the functionality of
[ComplexHeatmap::anno_summary()](https://jokergoo.github.io/ComplexHeatmap-reference/book/heatmap-annotations.html#summary-annotation),
but is versatile enough to be used with any heatmap, not just single-column or
single-row heatmaps.
```{r}
ggheatmap(small_mat) +
hmanno("t") +
align_dendro(aes(color = branch), k = 3L) +
scale_color_brewer(palette = "Dark2") +
hmanno("r", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(y = .extra_panel, x = value, fill = factor(.extra_panel))) +
# the default will always use continuous scale, here, we override it, since
# the panel is a factor variable, we should use discrete scale.
scale_y_discrete() +
scale_fill_brewer(palette = "Dark2", name = "branch") +
theme(axis.text.x = element_text(angle = -60, hjust = 0))
```
### Plot titles
`ggplot2` only allow add titles in the top or add caption in the bottom.
The ggalign package extends this capability, allowing you to place titles around
any border of the plot using the `patch_titles()` function.
```{r}
ggheatmap(small_mat) +
patch_titles(left = "left patch title", bottom = "bottom patch title") +
hmanno("t") +
align_dendro(aes(color = branch), k = 3L) +
scale_color_brewer(palette = "Dark2") +
patch_titles(top = "top patch title") +
hmanno("r", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(y = .extra_panel, x = value, fill = factor(.extra_panel))) +
scale_y_discrete() +
scale_fill_brewer(palette = "Dark2", name = "branch") +
theme(axis.text.x = element_text(angle = -60, hjust = 0)) +
patch_titles(right = "right patch title")
```
## `align_panel`
The `align_panel()` function is similar to align_gg(), but it operates
specifically with layout panel data, removing all axis labels and ticks. The
`ggpanel()` function is an alias for `align_panel()`.
The data in the underlying `ggplot` object contains following columns:
- `.panel`: the panel for current layout axis.
- `.index`: the index of the original layout data.
- `.x` or `.y`: the `x` or `y` coordinates
You can use `align_panel()` to integrate additional elements, such as block
annotation or customized panel title, into your layout.
```{r}
ggheatmap(small_mat) +
hmanno("t", size = unit(1, "cm")) +
align_kmeans(centers = 3L) +
ggpanel() +
geom_tile(aes(y = 1L, fill = .panel, color = .panel),
width = 1L, height = 1L
) +
geom_text(aes(y = 1L, label = .panel),
data = function(data) {
aggregate(.x ~ .panel, data, FUN = median)
}
)
```
## Session information
```{r}
sessionInfo()
```