Last updated on 2024-05-01 12:14:38 CEST.
| Flavor | Version | Tinstall | Tcheck | Ttotal | Status | Flags |
|---|---|---|---|---|---|---|
| r-devel-linux-x86_64-debian-clang | 0.2.6 | 80.09 | 838.48 | 918.57 | ERROR | |
| r-devel-linux-x86_64-debian-gcc | 0.2.6 | 59.65 | 1003.07 | 1062.72 | ERROR | |
| r-devel-linux-x86_64-fedora-clang | 0.2.6 | 1148.27 | ERROR | |||
| r-devel-linux-x86_64-fedora-gcc | 0.2.6 | 2065.44 | NOTE | |||
| r-devel-windows-x86_64 | 0.2.6 | 66.00 | 531.00 | 597.00 | NOTE | |
| r-patched-linux-x86_64 | 0.2.6 | 75.34 | 2377.84 | 2453.18 | NOTE | |
| r-release-linux-x86_64 | 0.2.6 | 76.99 | 1312.59 | 1389.58 | ERROR | |
| r-release-macos-arm64 | 0.2.6 | 369.00 | NOTE | |||
| r-release-macos-x86_64 | 0.2.6 | 1070.00 | NOTE | |||
| r-release-windows-x86_64 | 0.2.6 | 74.00 | 538.00 | 612.00 | NOTE | |
| r-oldrel-macos-arm64 | 0.2.6 | 411.00 | NOTE | |||
| r-oldrel-macos-x86_64 | 0.2.6 | 849.00 | NOTE | |||
| r-oldrel-windows-x86_64 | 0.2.6 | 86.00 | 1134.00 | 1220.00 | NOTE |
Version: 0.2.6
Check: for GNU extensions in Makefiles
Result: NOTE
GNU make is a SystemRequirements.
Flavors: r-devel-linux-x86_64-debian-clang, r-devel-linux-x86_64-debian-gcc, r-devel-linux-x86_64-fedora-clang, r-devel-linux-x86_64-fedora-gcc, r-devel-windows-x86_64, r-patched-linux-x86_64, r-release-linux-x86_64, r-release-macos-arm64, r-release-macos-x86_64, r-release-windows-x86_64, r-oldrel-macos-arm64, r-oldrel-macos-x86_64, r-oldrel-windows-x86_64
Version: 0.2.6
Check: tests
Result: ERROR
Running ‘test_RadixForest.R’ [17s/21s]
Running ‘test_RadixTree.R’ [642s/395s]
Running ‘test_pairwise.R’ [122s/88s]
Running the tests in ‘tests/test_pairwise.R’ failed.
Complete output:
> # This test file tests the `dist_matrix` and `dist_pairwise` functions
> # These two functions are simple dynamic programming algorithms for computing pairwise distances and are themselves used to validate
> # the RadixTree imeplementation (see test_radix_tree.R)
>
> library(seqtrie)
> library(stringi)
> library(stringdist)
> library(Biostrings)
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, table, tapply,
union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':
findMatches
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
> library(dplyr)
Attaching package: 'dplyr'
The following objects are masked from 'package:Biostrings':
collapse, intersect, setdiff, setequal, union
The following object is masked from 'package:GenomeInfoDb':
intersect
The following object is masked from 'package:XVector':
slice
The following objects are masked from 'package:IRanges':
collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':
first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':
combine, intersect, setdiff, union
The following objects are masked from 'package:stats':
filter, lag
The following objects are masked from 'package:base':
intersect, setdiff, setequal, union
>
> # Use 2 threads on github actions and CRAN, 4 threads locally
> IS_LOCAL <- Sys.getenv("IS_LOCAL") != ""
> NTHREADS <- ifelse(IS_LOCAL, 4, 2)
> NITER <- ifelse(IS_LOCAL, 4, 1)
> NSEQS <- 10000
> MAXSEQLEN <- 200
> CHARSET <- "ACGT"
>
> random_strings <- function(N, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset_stri <- paste0("[", charset, "]")
+ len <- sample(0:MAXSEQLEN, N, replace=TRUE)
+ result <- lapply(0:MAXSEQLEN, function(x) {
+ nx <- sum(len == x)
+ if(nx == 0) return(character())
+ stringi::stri_rand_strings(nx, x, pattern = charset_stri)
+ })
+ sample(unlist(result))
+ }
>
> mutate_strings <- function(x, prob = 0.025, indel_prob = 0.025, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset <- unlist(strsplit(charset, ""))
+ xsplit <- strsplit(x, "")
+ sapply(xsplit, function(a) {
+ r <- runif(length(a)) < prob
+ a[r] <- sample(charset, sum(r), replace=TRUE)
+ ins <- runif(length(a)) < indel_prob
+ a[ins] <- paste0(sample(charset, sum(ins), replace=TRUE), sample(charset, sum(ins), replace=TRUE))
+ del <- runif(length(a)) < indel_prob
+ a[del] <- ""
+ paste0(a, collapse = "")
+ })
+ }
>
> # Biostrings notes:
> # subject (target) must be of length 1 or equal to pattern (query)
> # To get a distance matrix, iterate over target and perform a column bind
> # special_zero_case -- if both query and target are empty, Biostrings fails with an error
> pairwiseAlignmentFix <- function(pattern, subject, ...) {
+ results <- rep(0, length(subject))
+ special_zero_case <- nchar(pattern) == 0 & nchar(subject) == 0
+ if(all(special_zero_case)) {
+ results
+ } else {
+ results[!special_zero_case] <- Biostrings::pairwiseAlignment(pattern=pattern[!special_zero_case], subject=subject[!special_zero_case], ...)
+ results
+ }
+ }
>
> biostrings_matrix_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(query, function(x) {
+ query2 <- rep(x, length(target))
+ -pairwiseAlignmentFix(pattern=query2, subject=target, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ -pairwiseAlignment(pattern=query, subject=target, substitutionMatrix = substitutionMatrix,gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> biostrings_matrix_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(seq_along(query), function(i) {
+ query2 <- substring(query[i], 1, query_size[i,,drop=TRUE])
+ target2 <- substring(target, 1, target_size[i,,drop=TRUE])
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ query2 <- substring(query, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> for(. in 1:NITER) {
+
+ print("Checking hamming search correctness")
+ local({
+ # Note: seqtrie returns `NA_integer_` for hamming distance when the lengths are different
+ # whereas stringdist returns `Inf`
+ # This is why we need to replace `NA_integer_` with `Inf` when comparing results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking levenshtein search correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking anchored search correctness")
+ local({
+ # There is no anchored search in stringdist (or elsewhere). To get the same results, we substring the query and target sequences
+ # By the results of the seqtrie anchored search and then compare the results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_stringdist <- lapply(seq_along(query), function(i) {
+ query_size2 <- query_size[i,,drop=TRUE]
+ target_size2 <- target_size[i,,drop=TRUE]
+ query2 <- substring(query[i], 1, query_size2) # query[i] is recycled
+ target2 <- substring(target, 1, target_size2)
+ stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ }) %>% do.call(rbind, .)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ query2 <- substring(query_pairwise, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ results_stringdist <- stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking global search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+
+ print("Checking global search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cos=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+ }
[1] "Checking hamming search correctness"
[1] "Checking levenshtein search correctness"
[1] "Checking anchored search correctness"
[1] "Checking global search with linear gap for correctness"
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'args' in selecting a method for function 'do.call': Could not load package pwalign. Is it installed?
Note that Starting with BioC 3.19, calling pairwiseAlignment() requires
the pwalign package. Please install it with:
BiocManager::install("pwalign")
Calls: <Anonymous> ... <Anonymous> -> .call_fun_in_pwalign -> .load_package_gracefully
Execution halted
Flavor: r-devel-linux-x86_64-debian-clang
Version: 0.2.6
Check: tests
Result: ERROR
Running ‘test_RadixForest.R’ [18s/21s]
Running ‘test_RadixTree.R’ [13m/10m]
Running ‘test_pairwise.R’ [147s/126s]
Running the tests in ‘tests/test_pairwise.R’ failed.
Complete output:
> # This test file tests the `dist_matrix` and `dist_pairwise` functions
> # These two functions are simple dynamic programming algorithms for computing pairwise distances and are themselves used to validate
> # the RadixTree imeplementation (see test_radix_tree.R)
>
> library(seqtrie)
> library(stringi)
> library(stringdist)
> library(Biostrings)
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, table, tapply,
union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':
findMatches
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
> library(dplyr)
Attaching package: 'dplyr'
The following objects are masked from 'package:Biostrings':
collapse, intersect, setdiff, setequal, union
The following object is masked from 'package:GenomeInfoDb':
intersect
The following object is masked from 'package:XVector':
slice
The following objects are masked from 'package:IRanges':
collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':
first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':
combine, intersect, setdiff, union
The following objects are masked from 'package:stats':
filter, lag
The following objects are masked from 'package:base':
intersect, setdiff, setequal, union
>
> # Use 2 threads on github actions and CRAN, 4 threads locally
> IS_LOCAL <- Sys.getenv("IS_LOCAL") != ""
> NTHREADS <- ifelse(IS_LOCAL, 4, 2)
> NITER <- ifelse(IS_LOCAL, 4, 1)
> NSEQS <- 10000
> MAXSEQLEN <- 200
> CHARSET <- "ACGT"
>
> random_strings <- function(N, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset_stri <- paste0("[", charset, "]")
+ len <- sample(0:MAXSEQLEN, N, replace=TRUE)
+ result <- lapply(0:MAXSEQLEN, function(x) {
+ nx <- sum(len == x)
+ if(nx == 0) return(character())
+ stringi::stri_rand_strings(nx, x, pattern = charset_stri)
+ })
+ sample(unlist(result))
+ }
>
> mutate_strings <- function(x, prob = 0.025, indel_prob = 0.025, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset <- unlist(strsplit(charset, ""))
+ xsplit <- strsplit(x, "")
+ sapply(xsplit, function(a) {
+ r <- runif(length(a)) < prob
+ a[r] <- sample(charset, sum(r), replace=TRUE)
+ ins <- runif(length(a)) < indel_prob
+ a[ins] <- paste0(sample(charset, sum(ins), replace=TRUE), sample(charset, sum(ins), replace=TRUE))
+ del <- runif(length(a)) < indel_prob
+ a[del] <- ""
+ paste0(a, collapse = "")
+ })
+ }
>
> # Biostrings notes:
> # subject (target) must be of length 1 or equal to pattern (query)
> # To get a distance matrix, iterate over target and perform a column bind
> # special_zero_case -- if both query and target are empty, Biostrings fails with an error
> pairwiseAlignmentFix <- function(pattern, subject, ...) {
+ results <- rep(0, length(subject))
+ special_zero_case <- nchar(pattern) == 0 & nchar(subject) == 0
+ if(all(special_zero_case)) {
+ results
+ } else {
+ results[!special_zero_case] <- Biostrings::pairwiseAlignment(pattern=pattern[!special_zero_case], subject=subject[!special_zero_case], ...)
+ results
+ }
+ }
>
> biostrings_matrix_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(query, function(x) {
+ query2 <- rep(x, length(target))
+ -pairwiseAlignmentFix(pattern=query2, subject=target, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ -pairwiseAlignment(pattern=query, subject=target, substitutionMatrix = substitutionMatrix,gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> biostrings_matrix_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(seq_along(query), function(i) {
+ query2 <- substring(query[i], 1, query_size[i,,drop=TRUE])
+ target2 <- substring(target, 1, target_size[i,,drop=TRUE])
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ query2 <- substring(query, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> for(. in 1:NITER) {
+
+ print("Checking hamming search correctness")
+ local({
+ # Note: seqtrie returns `NA_integer_` for hamming distance when the lengths are different
+ # whereas stringdist returns `Inf`
+ # This is why we need to replace `NA_integer_` with `Inf` when comparing results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking levenshtein search correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking anchored search correctness")
+ local({
+ # There is no anchored search in stringdist (or elsewhere). To get the same results, we substring the query and target sequences
+ # By the results of the seqtrie anchored search and then compare the results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_stringdist <- lapply(seq_along(query), function(i) {
+ query_size2 <- query_size[i,,drop=TRUE]
+ target_size2 <- target_size[i,,drop=TRUE]
+ query2 <- substring(query[i], 1, query_size2) # query[i] is recycled
+ target2 <- substring(target, 1, target_size2)
+ stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ }) %>% do.call(rbind, .)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ query2 <- substring(query_pairwise, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ results_stringdist <- stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking global search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+
+ print("Checking global search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cos=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+ }
[1] "Checking hamming search correctness"
[1] "Checking levenshtein search correctness"
[1] "Checking anchored search correctness"
[1] "Checking global search with linear gap for correctness"
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'args' in selecting a method for function 'do.call': Could not load package pwalign. Is it installed?
Note that Starting with BioC 3.19, calling pairwiseAlignment() requires
the pwalign package. Please install it with:
BiocManager::install("pwalign")
Calls: <Anonymous> ... <Anonymous> -> .call_fun_in_pwalign -> .load_package_gracefully
Execution halted
Flavor: r-devel-linux-x86_64-debian-gcc
Version: 0.2.6
Check: tests
Result: ERROR
Running ‘test_RadixForest.R’ [26s/21s]
Running ‘test_RadixTree.R’ [746s/563s]
Running ‘test_pairwise.R’ [185s/159s]
Running the tests in ‘tests/test_pairwise.R’ failed.
Complete output:
> # This test file tests the `dist_matrix` and `dist_pairwise` functions
> # These two functions are simple dynamic programming algorithms for computing pairwise distances and are themselves used to validate
> # the RadixTree imeplementation (see test_radix_tree.R)
>
> library(seqtrie)
> library(stringi)
> library(stringdist)
> library(Biostrings)
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, table, tapply,
union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':
findMatches
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
> library(dplyr)
Attaching package: 'dplyr'
The following objects are masked from 'package:Biostrings':
collapse, intersect, setdiff, setequal, union
The following object is masked from 'package:GenomeInfoDb':
intersect
The following object is masked from 'package:XVector':
slice
The following objects are masked from 'package:IRanges':
collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':
first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':
combine, intersect, setdiff, union
The following objects are masked from 'package:stats':
filter, lag
The following objects are masked from 'package:base':
intersect, setdiff, setequal, union
>
> # Use 2 threads on github actions and CRAN, 4 threads locally
> IS_LOCAL <- Sys.getenv("IS_LOCAL") != ""
> NTHREADS <- ifelse(IS_LOCAL, 4, 2)
> NITER <- ifelse(IS_LOCAL, 4, 1)
> NSEQS <- 10000
> MAXSEQLEN <- 200
> CHARSET <- "ACGT"
>
> random_strings <- function(N, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset_stri <- paste0("[", charset, "]")
+ len <- sample(0:MAXSEQLEN, N, replace=TRUE)
+ result <- lapply(0:MAXSEQLEN, function(x) {
+ nx <- sum(len == x)
+ if(nx == 0) return(character())
+ stringi::stri_rand_strings(nx, x, pattern = charset_stri)
+ })
+ sample(unlist(result))
+ }
>
> mutate_strings <- function(x, prob = 0.025, indel_prob = 0.025, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset <- unlist(strsplit(charset, ""))
+ xsplit <- strsplit(x, "")
+ sapply(xsplit, function(a) {
+ r <- runif(length(a)) < prob
+ a[r] <- sample(charset, sum(r), replace=TRUE)
+ ins <- runif(length(a)) < indel_prob
+ a[ins] <- paste0(sample(charset, sum(ins), replace=TRUE), sample(charset, sum(ins), replace=TRUE))
+ del <- runif(length(a)) < indel_prob
+ a[del] <- ""
+ paste0(a, collapse = "")
+ })
+ }
>
> # Biostrings notes:
> # subject (target) must be of length 1 or equal to pattern (query)
> # To get a distance matrix, iterate over target and perform a column bind
> # special_zero_case -- if both query and target are empty, Biostrings fails with an error
> pairwiseAlignmentFix <- function(pattern, subject, ...) {
+ results <- rep(0, length(subject))
+ special_zero_case <- nchar(pattern) == 0 & nchar(subject) == 0
+ if(all(special_zero_case)) {
+ results
+ } else {
+ results[!special_zero_case] <- Biostrings::pairwiseAlignment(pattern=pattern[!special_zero_case], subject=subject[!special_zero_case], ...)
+ results
+ }
+ }
>
> biostrings_matrix_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(query, function(x) {
+ query2 <- rep(x, length(target))
+ -pairwiseAlignmentFix(pattern=query2, subject=target, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ -pairwiseAlignment(pattern=query, subject=target, substitutionMatrix = substitutionMatrix,gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> biostrings_matrix_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(seq_along(query), function(i) {
+ query2 <- substring(query[i], 1, query_size[i,,drop=TRUE])
+ target2 <- substring(target, 1, target_size[i,,drop=TRUE])
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ query2 <- substring(query, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> for(. in 1:NITER) {
+
+ print("Checking hamming search correctness")
+ local({
+ # Note: seqtrie returns `NA_integer_` for hamming distance when the lengths are different
+ # whereas stringdist returns `Inf`
+ # This is why we need to replace `NA_integer_` with `Inf` when comparing results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking levenshtein search correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking anchored search correctness")
+ local({
+ # There is no anchored search in stringdist (or elsewhere). To get the same results, we substring the query and target sequences
+ # By the results of the seqtrie anchored search and then compare the results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_stringdist <- lapply(seq_along(query), function(i) {
+ query_size2 <- query_size[i,,drop=TRUE]
+ target_size2 <- target_size[i,,drop=TRUE]
+ query2 <- substring(query[i], 1, query_size2) # query[i] is recycled
+ target2 <- substring(target, 1, target_size2)
+ stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ }) %>% do.call(rbind, .)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ query2 <- substring(query_pairwise, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ results_stringdist <- stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking global search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+
+ print("Checking global search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cos=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+ }
[1] "Checking hamming search correctness"
[1] "Checking levenshtein search correctness"
[1] "Checking anchored search correctness"
[1] "Checking global search with linear gap for correctness"
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'args' in selecting a method for function 'do.call': Could not load package pwalign. Is it installed?
Note that Starting with BioC 3.19, calling pairwiseAlignment() requires
the pwalign package. Please install it with:
BiocManager::install("pwalign")
Calls: <Anonymous> ... <Anonymous> -> .call_fun_in_pwalign -> .load_package_gracefully
Execution halted
Flavor: r-devel-linux-x86_64-fedora-clang
Version: 0.2.6
Check: tests
Result: ERROR
Running ‘test_RadixForest.R’ [34s/33s]
Running ‘test_RadixTree.R’ [17m/10m]
Running ‘test_pairwise.R’ [217s/149s]
Running the tests in ‘tests/test_pairwise.R’ failed.
Complete output:
> # This test file tests the `dist_matrix` and `dist_pairwise` functions
> # These two functions are simple dynamic programming algorithms for computing pairwise distances and are themselves used to validate
> # the RadixTree imeplementation (see test_radix_tree.R)
>
> library(seqtrie)
> library(stringi)
> library(stringdist)
> library(Biostrings)
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, table, tapply,
union, unique, unsplit, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':
findMatches
The following objects are masked from 'package:base':
I, expand.grid, unname
Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
> library(dplyr)
Attaching package: 'dplyr'
The following objects are masked from 'package:Biostrings':
collapse, intersect, setdiff, setequal, union
The following object is masked from 'package:GenomeInfoDb':
intersect
The following object is masked from 'package:XVector':
slice
The following objects are masked from 'package:IRanges':
collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':
first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':
combine, intersect, setdiff, union
The following objects are masked from 'package:stats':
filter, lag
The following objects are masked from 'package:base':
intersect, setdiff, setequal, union
>
> # Use 2 threads on github actions and CRAN, 4 threads locally
> IS_LOCAL <- Sys.getenv("IS_LOCAL") != ""
> NTHREADS <- ifelse(IS_LOCAL, 4, 2)
> NITER <- ifelse(IS_LOCAL, 4, 1)
> NSEQS <- 10000
> MAXSEQLEN <- 200
> CHARSET <- "ACGT"
>
> random_strings <- function(N, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset_stri <- paste0("[", charset, "]")
+ len <- sample(0:MAXSEQLEN, N, replace=TRUE)
+ result <- lapply(0:MAXSEQLEN, function(x) {
+ nx <- sum(len == x)
+ if(nx == 0) return(character())
+ stringi::stri_rand_strings(nx, x, pattern = charset_stri)
+ })
+ sample(unlist(result))
+ }
>
> mutate_strings <- function(x, prob = 0.025, indel_prob = 0.025, charset = "abcdefghijklmnopqrstuvwxyz") {
+ charset <- unlist(strsplit(charset, ""))
+ xsplit <- strsplit(x, "")
+ sapply(xsplit, function(a) {
+ r <- runif(length(a)) < prob
+ a[r] <- sample(charset, sum(r), replace=TRUE)
+ ins <- runif(length(a)) < indel_prob
+ a[ins] <- paste0(sample(charset, sum(ins), replace=TRUE), sample(charset, sum(ins), replace=TRUE))
+ del <- runif(length(a)) < indel_prob
+ a[del] <- ""
+ paste0(a, collapse = "")
+ })
+ }
>
> # Biostrings notes:
> # subject (target) must be of length 1 or equal to pattern (query)
> # To get a distance matrix, iterate over target and perform a column bind
> # special_zero_case -- if both query and target are empty, Biostrings fails with an error
> pairwiseAlignmentFix <- function(pattern, subject, ...) {
+ results <- rep(0, length(subject))
+ special_zero_case <- nchar(pattern) == 0 & nchar(subject) == 0
+ if(all(special_zero_case)) {
+ results
+ } else {
+ results[!special_zero_case] <- Biostrings::pairwiseAlignment(pattern=pattern[!special_zero_case], subject=subject[!special_zero_case], ...)
+ results
+ }
+ }
>
> biostrings_matrix_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(query, function(x) {
+ query2 <- rep(x, length(target))
+ -pairwiseAlignmentFix(pattern=query2, subject=target, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_global <- function(query, target, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ -pairwiseAlignment(pattern=query, subject=target, substitutionMatrix = substitutionMatrix,gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> biostrings_matrix_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ lapply(seq_along(query), function(i) {
+ query2 <- substring(query[i], 1, query_size[i,,drop=TRUE])
+ target2 <- substring(target, 1, target_size[i,,drop=TRUE])
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }) %>% do.call(rbind, .)
+ }
>
> biostrings_pairwise_anchored <- function(query, target, query_size, target_size, cost_matrix, gap_cost, gap_open_cost = 0) {
+ substitutionMatrix <- -cost_matrix
+ query2 <- substring(query, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ -pairwiseAlignmentFix(pattern=query2, subject=target2, substitutionMatrix = substitutionMatrix, gapOpening=gap_open_cost, gapExtension=gap_cost, scoreOnly=TRUE, type="global")
+ }
>
> for(. in 1:NITER) {
+
+ print("Checking hamming search correctness")
+ local({
+ # Note: seqtrie returns `NA_integer_` for hamming distance when the lengths are different
+ # whereas stringdist returns `Inf`
+ # This is why we need to replace `NA_integer_` with `Inf` when comparing results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "hamming", nthreads=NTHREADS)
+ results_seqtrie[is.na(results_seqtrie)] <- Inf
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "hamming", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking levenshtein search correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdistmatrix(query, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", nthreads=NTHREADS)
+ results_stringdist <- stringdist::stringdist(query_pairwise, target, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking anchored search correctness")
+ local({
+ # There is no anchored search in stringdist (or elsewhere). To get the same results, we substring the query and target sequences
+ # By the results of the seqtrie anchored search and then compare the results
+
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_stringdist <- lapply(seq_along(query), function(i) {
+ query_size2 <- query_size[i,,drop=TRUE]
+ target_size2 <- target_size[i,,drop=TRUE]
+ query2 <- substring(query[i], 1, query_size2) # query[i] is recycled
+ target2 <- substring(target, 1, target_size2)
+ stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ }) %>% do.call(rbind, .)
+ stopifnot(all(results_seqtrie == results_stringdist))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ query2 <- substring(query_pairwise, 1, query_size)
+ target2 <- substring(target, 1, target_size)
+ results_stringdist <- stringdist::stringdist(query2, target2, method = "lv", nthread=NTHREADS)
+ stopifnot(all(results_seqtrie == results_stringdist))
+ })
+
+ print("Checking global search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with linear gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+
+ print("Checking global search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cos=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_matrix_global(query, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "levenshtein", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ results_biostrings <- biostrings_pairwise_global(query_pairwise, target, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+
+ print("Checking anchored search with affine gap for correctness")
+ local({
+ target <- c(random_strings(NSEQS, CHARSET),"") %>% unique
+ query <- sample(c(sample(target, NSEQS/1000), random_strings(NSEQS/1000, CHARSET)))
+ query <- c(mutate_strings(query, indel_prob=0, charset = CHARSET), "") %>% unique
+
+ # Check matrix results
+ cost_matrix <- matrix(sample(1:3, size = nchar(CHARSET)^2, replace=TRUE), nrow=nchar(CHARSET))
+ diag(cost_matrix) <- 0
+ colnames(cost_matrix) <- rownames(cost_matrix) <- strsplit(CHARSET, "")[[1]]
+ gap_cost <- sample(1:3, size = 1)
+ gap_open_cost <- sample(1:3, size = 1)
+ results_seqtrie <- dist_matrix(query, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_matrix_anchored(query, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+
+ # Check pairwise results
+ query_pairwise <- mutate_strings(target, prob=0.025, indel_prob=0.05, charset = CHARSET)
+ results_seqtrie <- dist_pairwise(query_pairwise, target, mode = "anchored", cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost, nthreads=NTHREADS)
+ query_size <- attr(results_seqtrie, "query_size")
+ target_size <- attr(results_seqtrie, "target_size")
+ results_biostrings <- biostrings_pairwise_anchored(query_pairwise, target, query_size, target_size, cost_matrix = cost_matrix, gap_cost = gap_cost, gap_open_cost=gap_open_cost)
+ stopifnot(all(results_seqtrie == results_biostrings))
+ })
+ }
[1] "Checking hamming search correctness"
[1] "Checking levenshtein search correctness"
[1] "Checking anchored search correctness"
[1] "Checking global search with linear gap for correctness"
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'args' in selecting a method for function 'do.call': Could not load package pwalign. Is it installed?
Note that Starting with BioC 3.19, calling pairwiseAlignment() requires
the pwalign package. Please install it with:
BiocManager::install("pwalign")
Calls: <Anonymous> ... <Anonymous> -> .call_fun_in_pwalign -> .load_package_gracefully
Execution halted
Flavor: r-release-linux-x86_64
Version: 0.2.6
Check: installed package size
Result: NOTE
installed size is 5.8Mb
sub-directories of 1Mb or more:
data 1.1Mb
libs 4.2Mb
Flavors: r-release-macos-arm64, r-release-macos-x86_64, r-oldrel-macos-arm64, r-oldrel-macos-x86_64